Competent Cells
MilliporeSigma™ Novagen™ NovaBlue Competent Cells
NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.
MilliporeSigma™ Novagen™ DE3 Competent Cell Set
For Protein Expressions
Invitrogen™ MultiShot™ Stbl3™Chemically Competent E. coli Cells, 96-tube rack
MultiShot Stbl3 Chemically Competent E. coli Cells support cloning of unstable or repetitive DNA with flexible workflow capacity. The 96-tube rack format allows scalable, high-throughput cloning while maintaining construct stability and integrity.
MilliporeSigma™ Novagen™ Tuner™ (DE3) pLacI Competent Cells
Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. Contains the pLacI plasmid producing extra Lac repressor.
MilliporeSigma™ Novagen™ Rosetta-gami™ 2 (DE3) Competent Cells
Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
MilliporeSigma™ Novagen™ BL21 (DE3) pLysS Singles™ Competent Cells
BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.
MilliporeSigma™ Novagen™ BL21 Competent Cell Set
BL21 is the most widely used host background and has the advantage of being deficient in both lon (1) and ompT proteases.
Invitrogen™ MultiShot™ StripWell Stbl3™ Competent Cells
MultiShot StripWell Stbl3 Chemically Competent E. coli cells are cloning competent cells designed for cloning direct repeats found in lentiviral expression vectors.
Invitrogen™ ElectroMAX™ DH5α-E Competent Cells
ElectroMAX DH5α-E Competent Cells are derived from the DH5α strain and are suitable for transformation by electroporation.
Invitrogen™ One Shot™ INVαF' Chemically Competent E. coli
One Shot INVαF' Chemically Competent E. coli are designed to stably replicate high-copy number plasmids.
MilliporeSigma™ Novagen™ Origami™ 2 (DE3) Competent Cells
Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.
MilliporeSigma™ Novagen™ Origami™ 2 (DE3) pLysS Competent Cells
Origami 2 strains have mutations in glutathione reducatase(gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation. T7 lysozyme expression suppresses basal T7 expression.
MilliporeSigma™ Novagen™ HMS174 Competent Cells
HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.
MilliporeSigma™ Novagen™ NovaBlue(DE3) Competent Cells
NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.
MilliporeSigma™ Novagen™ Rosetta-gami™ 2 Competent Cell Set
Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.